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From virus deactivation in-vitro to clinical relevance: a glycerol and cod trypsin-containing mouth spray in the common cold

EFSM: 2021;1:210018DOI: 10.52778/efsm.21.0018Publikováno dne: 16.08.2021
Tanja Schütt a Matthias Löhn

Trypsin combined with glycerol caused the deactivation of common cold viruses in vitro. A pilot study and a comparative multicentre study investigated the in vivo reduction in viral load and the improvement in quality of life after use of the active substances as a throat spray by subjects suffering from the common cold. The results showed a decrease in viral load, a shortening of the duration of the cold and an improvement in the quality of life.

With the beginning of autumn, the common cold season starts – runny and stuffy nose, dry and chesty cough, sometimes accompanied by fever, headache or body pain – a daily routine in pharmacies. As causal treatments are missing, recommendation follows individual symptom relief. All symptoms are a sign for an activated immune system with a clear objective – fighting against the common cold virus. Means in turn, less virus – less symptoms. One approach pays to this goal: support the immune system by getting rid of the virus as source of the problem.

Virus deactivation – interesting approach in common cold treatment

Cod trypsin is a serine protease from cold water fish with anti-inflammatory properties [1]. The enzyme combined with glycerol has shown a deactivation of several common cold viruses in-vitro (table) [2, 3]. Due to the broad variety of vulnerable viruses it became an interesting candidate for the early treatment of common cold, considering that over 200 serologically different viral types are responsible for human URTIs, with the rhinoviruses being the most common cause [4]. For this reason, it leads to 2–4 common cold episode per year in adults and even 6–8 episode per year in children [5].

From in vitro to in vivo: Application via mouth spray – 6 times daily

Although Rhinovirus is described to replicate best at 33°C [7], it has been found in the lower respiratory tract [7]. This might be the byproduct of fast muco-ciliary transport and cleaning of the nose before virus inoculation and the increase of mouth breathing after virus inoculation due to a stuffy nose. The oropharynx as gate to the lower respiratory tract seems to be a zone where everything comes together and where a protective, virus trapping layer of Cod trypsin and glycerol would make a lot of sense. The physiological “washing” is the applicationfrequency-defining-step, because the half-life activity of cod trypsin is quite long at human body temperature of about 0.7 days [8].

Clinical relevance in common cold: Rhinoviral load reduction in the oropharynx by more than 99% accompanied by significant reduction of symptom severity, halving the duration of a common cold and increase of quality of life of common cold patients

Successful pilot study in 46 adult healthy volunteer’s received rhinovirus-16 and an infection rate of 76% was achieved. Treatment with the mouth spray 6 times daily for 10 days led to a significantly reduced total viral load (median of 7.4 × 103 copies/mL) in the oropharynx compared to the placebo group (6.3 × 1011 copies/mL; p = 0.023), which corresponds to a viral load reduction by more than 99% [8]. Furthermore, the number of days with common cold symptoms has been significantly reduced by the mouth spray from 6.5 to 3.0 days (p = 0.014) [9].

Confirmatory prospective, randomized parallel group study in 267 subjects with naturally acquired colds. The efficacy of the mouth spray was evaluated for quality of life by the Wisconsin Upper Respiratory Symptom Survey-21 (WURSS-21 Quality of life scale), the Jackson score (a scoring system which assesses individuals’ subjective ratings of 8 respiratory symptoms), and for the Quality of Life (QoL) component [10]. All three symptom scales showed statistically significant improvement. Quality of life parameters were: “think clearly, sleep well, breathe easily, walk, climb stairs, exercise, accomplish daily activities, work outside and inside the home, interact with others, live your personal life.” Interestingly, the need for complementary use of symptom-relieving medication for colds was reduced [10].

Real world evidence: surveys revealed reduced number of sick days in different groups like competitive and endurance athletes, elderly care personnel or pre-school-staff. A common cold is not life threatening but sometimes it feels exactly like that because for sure the quality of life is dramatically impacted which goes hand in hand with absence from work and inability to make the day. Surveys of different designs revealed constantly a reduced number of sick days when the mouth spray has been applied early in the course of a common cold. Investigated groups were competitive athletes [11], endurance athletes [12, 13], pre-school staff [14] and elderly care personnel [15].


Cod trypsin / glycerol deactivated several common cold viruses in vitro which makes it to a promising candidate in the treatment of common cold. Applied as mouth-spray in vivo it reduced the Rhinovirus load in the oropharynx by more than 99%. Clinical trials brought the proof of concept and showed a significant reduction of common cold symptom severity with halving of the duration of a common cold accompanied by a significant increase of quality of life in several parameters. The convenient application via mouth spray offers an easy and hygienic common cold treatment even when suffering from a runny or stuffy nose, and even complementary to other symptomatic treatment. According to the positive outcomes of several real-world-survey’s different patient groups perceived a clear benefit. This leads inevitably to the question: Who is very often exposed to common cold viruses? Parents with smaller children, pharmacy staff… the list seems endless.


  1. Gudmundsdóttir A and Pálsdóttir HM. Atlantic cod trypsins: from basic research to practical applications. Mar Biotechnol (NY). 2005;7(2):77–88.
  2. Stefansson et al, A medical device forming a protective barrier that deactivates four major common cold viruses. Virology Research Reviews, 2017;1(5): 1–3.
  3. Stefansson B, Gudmundsdottir Á, Clarsund M. ColdZyme forms a protective barrier in the throat that deactivates five major common cold viruses. Swedish Otolaryngology Congress, Apr 2018. (accessed 25.09.2020)
  4. Eccles R. Understanding the symptoms of the common cold and influenza. Lancet Infect Dis. 2005;5(11):718–725.
  5. Heikkinen T, Järvinen A. The common cold. Lancet. 2003;361(9351):51–59.
  6. Common Human Coronaviruses. National Center for Immunization and Respiratory Diseases (NCIRD), Division of Viral Diseases; (accessed 25.09.2020)
  7. Greenberg SB. Update on Human Rhinovirus and Coronavirus Infections. Semin Respir Crit Care Med. 2016;37(4):555–571.
  8. Stefansson B, Helgadóttir L, Olafsdottir S, Gudmundsdottir A, Bjarnason JB. Characterization of cold-adapted Atlantic cod (Gadus morhua) trypsin I--kinetic parameters, autolysis and thermal stability. Comp Biochem Physiol B Biochem Mol Biol. 2010 Feb;155(2):186–94.
  9. Clarsund, M., Fornbacke, M., Uller, L., Johnston, S. and Emanuelsson, C. A Randomized, Double-Blind, Placebo-Controlled Pilot Clinical Study on ColdZyme® Mouth Spray against Rhinovirus-Induced Common Cold. Open Journal of Respiratory Diseases 2017;7:125–135.
  10. Lindberg F. Multi-symptom Relief and Improvement of Quality of Life - A Comparative Multicenter Trial on ColdZyme® Mouth Spray in Common Cold. Presentation at the Swedish ENT days 2009 and (accessed 09/2020)
  11. Blom, U. and Nelson, I. User Experience of ColdZyme Mouth Spray against Common Cold in Competitive Athletes. Open Journal of Respiratory Diseases 2018;8:13–20.
  12. Davison G. Pilot study: Does ColdZyme® mouth spray reduce upper respiratory tract infection incidence or duration in endurance athletes? J Otol Rhinol 2018, Vol. 7.
  13. Davison G. et al. ColdZyme® Mouth Spray reduces duration of upper respiratory tract infection symptoms in endurance athletes under free living conditions. European Journal of Sport Science, 2020
  14. Clarsund, M. Evaluation of ColdZyme Mouth Spray against Common Cold in Preschool Staff. Open Journal of Respiratory Diseases 2017;7:136–140.
  15. Clarsund, M. and Persson, C. Evaluation of ColdZyme Mouth Spray against Common Cold in Elderly Care Personnel. Open Journal of Respiratory Diseases 2017;7:12–17. doi: 10.4236/ojrd.2017.71002.

Conflict of interest: T. Schütt and M. Löhn are employees of Sanofi. 

Disclosure: Medical writing and publication funded by Sanofi Aventis Deutschland GmbH.

Pracoviště/Korespondenční adresa: Tanja Schütt, PhD, Consumer Healthcare Medical Affairs, Sanofi-Aventis Deutschland GmbH, Industriepark Hoechst, 65026 Frankfurt am Main, Germany a Matthias Löhn, PhD, Sanofi-Aventis Deutschland GmbH, Industriepark Hoechst, Frankfurt am Main, Germany
Odesláno dne: 20.11.2020Přijato dne: 03.01.2021Publikováno dne: 16.08.2021
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